Welker) This product is related to the following categories: High-Fidelity (HF®) Restriction Endonucleases Products, Restriction Endonucleases B, Time-Saver Qualified Restriction Enzymes Products This product can be used in the following applications: Restriction Enzyme Digestion coli strain that carries the cloned and modified BstEII-HF gene from Bacillus stearothermophilus ET (N. Note: Also available as a FastDigest enzyme for rapid DNA digestion. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. PRODUCT NAME PRODUCT NUMBER BUFFER HF FACTOR AgeI-HF R3552 CutSmart 8 AgeI R0552 1.1 ApoI-HF R3566 CutSmart 25 ApoI R0566 3. et al (2008) Nucleic Acids Reseach 36, e50. The resolution isĪt the single nucleotide level. Thermo Scientific NotI restriction enzyme recognizes GCGGCCGC sites and cuts best at 37☌ in O buffer (Isoschizomers: CciNI). HF ENZYMES 4 WHAT IS A HIGH-FIDELITY ENZYME Wild type enzymes were tested in supplied buffer for comparisons. Is determined by capillary electrophoresis and peak analysis. Restriction Enzyme Competitor Study: Nuclease ContaminationĮcoRI, NotI, and BamHI from multiple suppliers were tested in reactionsĬontaining a fluorescent labeled single stranded, double stranded blunt,ģ’overhang or 5’ overhang containing oligonucleotides. HF enzymes are all Time-Saver qualified and can therefore. HF enzymes also exhibit dramatically reduced star activity. High Fidelity (HF) Restriction Enzymes have 100 activity in rCutSmart Buffer single-buffer simplicity means more straightforward and streamlined sample processing. Of our HF restriction enzymes are shown below. NotI has a High Fidelity version NotI-HF ( NEB R3189 ).

Examples of nuclease contamination studies for some NEB extensively performs quality controls on all standard and high-fidelity Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer single-buffer simplicity means more straightforward and streamlined sample processing.

Neb noti hf